Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography.
J Zhejiang Univ Sci B
; 10(12): 877-82, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-19946951
ABSTRACT
A high-performance liquid chromatography (HPLC) system was used in the reversed phase mode for the determination of benzalkonium chloride (BKC) in azithromycin viscous ophthalmic drops. A Venusil-XBP(L)-C(18) (150 mmx4.6 mm, 5 microm) column was used at 50 degrees C. The mobile phase consisted of a mixture of methanol-potassium phosphate (165, v/v). Two sample preparation methods were compared. The results suggested that, compared with an extraction procedure, a deproteinization procedure was much quicker and more convenient. Using the deproteinization procedure for sample preparation, calibration curves were linear in the range 5.0 to approximately 50 microg/ml. The within-day and inter-day coefficients of variation were less than 10%. The average recoveries were determined as 96.70%, 98.52%, and 97.96% at concentrations of 10.0, 30.0, and 50.0 microg/ml, respectively. Variability in precision did not exceed 5%. In conclusion, this HPLC method using a simple sample treatment procedure appears suitable for monitoring BKC content in azithromycin viscous ophthalmic drops.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Soluções Oftálmicas
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Compostos de Benzalcônio
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Técnicas de Química Analítica
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Cromatografia Líquida de Alta Pressão
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Azitromicina
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article