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CD16/32-specific biotinylated 2.4G2 single-chain Fv complexed with avidin-FITC enhances FITC-specific humoral immune response in vivo in a CD16-dependent manner.
Angyal, Adrienn; Szekeres, Zsuzsanna; Balogh, Péter; Neer, Zsuzsa; Szarka, Eszter; Virag, Viktor; Medgyesi, David; Prechl, Jozsef; Sarmay, Gabriella.
Afiliação
  • Angyal A; Department of Immunology, Eotvos Lorand University, 1117 Budapest, Hungary.
Int Immunol ; 22(2): 71-80, 2010 Feb.
Article em En | MEDLINE | ID: mdl-19951957
Fcgamma receptors (FcgammaRs) play an essential role in the regulation of immune response due to their ability to bind immune complexes. Activating FcgammaRs may facilitate antigen presentation and dendritic-cell maturation, while in the late phase of the immune response, the inhibitory FcgammaRIIb may down-regulate B-cell activation upon cross-linking with activating receptors. In this study, we investigated the in vivo role of FcgammaRs on the modulation of humoral immune response. In order to get well-defined immune complexes that can bind to both the activating and the inhibitory FcgammaRs, we designed a mono-biotinylated single-chain fragment variable construct from the rat anti-mouse CD16/32 clone 2.4G2, linked to avidin-FITC, and tested its effect on the FITC-hapten-specific T-independent type 2 (TI-2) and T-dependent (TD) immune response. When injected intravenously in mice, the complex bound to a small portion of B220+, CD11b(high) and CD11c(high) cells and was localized in the spleen on marginal zone macrophages 15 min after treatment. When applied as a booster following primary immunization with TI-2 (FITC-dextran) or TD (FITC-keyhole limpet haemocyanin) antigens, the complex elevated the number of hapten-specific IgM/IgG-producing B cells. This effect was diminished in CD16KO mice, suggesting that the activating-type FcgammaRIII might be a key mediator of this mechanism.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Avidina / Fluoresceína-5-Isotiocianato / Receptores de IgG / Anticorpos de Cadeia Única / Imunidade Humoral / Células Produtoras de Anticorpos Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Avidina / Fluoresceína-5-Isotiocianato / Receptores de IgG / Anticorpos de Cadeia Única / Imunidade Humoral / Células Produtoras de Anticorpos Idioma: En Ano de publicação: 2010 Tipo de documento: Article