Detection of human herpesvirus-7 DNA in bronchoalveolar lavage.
Intervirology
; 53(2): 119-23, 2010.
Article
em En
| MEDLINE
| ID: mdl-19955817
ABSTRACT
OBJECTIVES:
Human herpesvirus-7 (HHV-7) is a highly seroprevalent virus that, following primary infection, establishes latency or persistence in some tissues, including lung. The aim of this study was to investigate the prevalence of HHV-7 in the lower respiratory tract of hospitalized adult patients.METHODS:
The prevalence of HHV-7 DNA was determined by quantitative real-time PCR in 212 bronchoalveolar lavage (BAL) samples obtained from 153 patients. The molecular epidemiology and clinical role of HHV-7 were evaluated.RESULTS:
HHV-7 DNA was positive in 44 of 212 specimens (20.7%), obtained from 40 of 153 patients (26.1%), in particular 22/68 (32.35%) and 18/86 (20.9%) in transplant and non-transplant patients, respectively (1 patient evaluated both before and after transplantation). No significant difference according to transplant condition or discharge diagnosis was found. Viral load was >100,000 genome equivalents/ml BAL in 6/22 (27.3%) transplant recipients and 4/18 (22.2%) non-transplant patients (p = n.s.).CONCLUSIONS:
The evaluation of HHV-7 DNA in BAL may be useful to investigate its potential role in lower respiratory tract infection, alone or in association with other viral and/or non-viral pathogens and to distinguish latency from reactivation.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Pneumonia Viral
/
Infecções Respiratórias
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DNA Viral
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Líquido da Lavagem Broncoalveolar
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Reação em Cadeia da Polimerase
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Herpesvirus Humano 7
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Infecções por Roseolovirus
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article