Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV.
BMC Immunol
; 10: 61, 2009 Dec 03.
Article
em En
| MEDLINE
| ID: mdl-19958537
ABSTRACT
BACKGROUND:
The spike (S) protein is a major structural glycoprotein of coronavirus (CoV), the causal agent of severe acute respiratory syndrome (SARS). The S protein is a potent target for SARS-specific cell-mediated immune responses. However, the mechanism CoV pathogenesis in SARS and the role of special CTLs in virus clearance are still largely uncharacterized. Here, we describe a study that leads to the identification of a novel HLA-A*0201-restricted epitope from conserved regions of S protein.RESULTS:
First, different SARS-CoV sequences were analyzed to predict eight candidate peptides from conserved regions of the S protein based upon HLA-A*0201 binding and proteosomal cleavage. Four of eight candidate peptides were tested by HLA-A*0201 binding assays. Among the four candidate peptides, Sp8 (S958-966, VLNDILSRL) induced specific CTLs both ex vivo in PBLs of healthy HLA-A2+ donors and in HLA-A2.1/Kb transgenic mice immunized with a plasmid encoding full-length S protein. The immunized mice released IFN-gamma and lysed target cells upon stimulation with Sp8 peptide-pulsed autologous dendritic cells in comparison to other candidates.CONCLUSION:
These results suggest that Sp8 is a naturally processed epitope. We propose that Sp8 epitope should help in the characterization of mechanisms of virus control and immunopathology in SARS-CoV infection.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Glicoproteínas de Membrana
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Linfócitos T Citotóxicos
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Antígenos HLA-A
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Proteínas do Envelope Viral
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Coronavirus
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Epitopos de Linfócito T
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article