Pemphigus vulgaris antigen mRNA quantification for the staging of sentinel lymph nodes in head and neck cancer.
Br J Cancer
; 102(1): 181-7, 2010 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-19997107
ABSTRACT
BACKGROUND:
Molecular diagnosis has been proposed to enhance the intra-operative diagnosis of sentinel lymph node (SLN) invasion in head and neck squamous cell carcinoma (HNSCC). Although cytokeratin (CK) mRNA quantification with real-time reverse transcriptase-PCR (QRT-PCR) has produced encouraging results, the more discriminating markers remain to be identified.METHODS:
Pemphigus vulgaris antigen (PVA), squamous cell carcinoma antigen (SCCA), and CK17 mRNA were quantified using QRT-PCR, and the results were compared with an extensive histopathological examination of the entire SLNs on 78 SLNs harvested from 22 patients with HNSCC.RESULTS:
SCCA and CK17 quantification showed significantly higher mRNA values for macrometastases (MAs) than for either negative or isolated tumour cell (ITC) SLNs (P<0.01). Pemphigus vulgaris antigen allowed the discrimination of all MAs and micrometastases from both negative and ITC SLNs (P<0.001). For the neck staging of patients, considering metastatic vs non-metastatic status, receiver-operating characteristic curve analysis found areas under the curve of 93.8, 97.9, and 100% for CK17, SCCA, and PVA, respectively. With PVA, a cutoff value of 562 copies per 100 ng of cDNA permitted the correct distinction between patients with positive as opposed to negative neck nodes in all cases.CONCLUSION:
PVA seems to be a highly promising marker for accurate intra-operative SLN staging in HNSCC by QRT-PCR.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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RNA Neoplásico
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Neoplasias da Língua
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Carcinoma de Células Escamosas
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Neoplasias Orofaríngeas
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Biomarcadores Tumorais
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Desmogleína 3
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Metástase Linfática
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Antígenos de Neoplasias
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Estadiamento de Neoplasias
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article