Simultaneous determination of five-type hepatitis virus antigens in 5 min using an integrated automatic electrochemical immunosensor array.
Biosens Bioelectron
; 25(7): 1658-62, 2010 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-20022741
ABSTRACT
An integrated automatic electrochemical immunosensor array has been designed for the simultaneous detection of 5-type hepatitis virus antigens (i.e. hepatitis A, hepatitis B, hepatitis C, hepatitis D, and hepatitis E). Initially, 5-type hepatitis virus antibodies were immobilized onto a self-made electrochemical sensor array using nanogold particles and protein A as matrices, and the immunosensor array was then used to capture their corresponding antigens from sample solution with a 1-step capture format. The detection is based on the potential change before and after the antigen-antibody reaction by using a 2-electrode system. The developed immunosensor array with allowed simultaneous determination of 5-type hepatitis virus antigens in 5 min. The detection limit of the sensor array was
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Robótica
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Imunoensaio
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Técnicas Biossensoriais
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Condutometria
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Antígenos de Hepatite
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Análise em Microsséries
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article