[Construction and expression of hepatitis B virus envelope protein combined with core protein with two multiple cloning sites vector].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 23(4): 313-5, 2009 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-20108784
ABSTRACT
OBJECTIVE:
To develop a coexpression plasmid which expressing envelope protein and nucleoprotein of hepatitis B virus and know of its expressing efficiency.METHODS:
The plasmid coexpressing envelope protein and nucleoprotein of hepatitis B virus under the CMV promoter respectively was constructed by gene recombination. Cellular expression was assessed by ELISA.RESULTS:
Multiple cloning site was inserted into expression vector contain hepatitis B virus PreS2-S gene. And expression unit containing hepatitis B virus PreC-C was cloned into it. HBsAg and HBeAg was detected both in the culture supernatant and in the cells.CONCLUSION:
The coexpressing plasmid was constructed successfully and it can express effectively in vitro. This has provided a basis for further research of the therapeutic HBV DNA vaccine.
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Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Clonagem Molecular
/
Antígenos do Núcleo do Vírus da Hepatite B
/
Antígenos de Superfície da Hepatite B
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article