Involvement of the nitric oxide/(C)GMP/K(ATP) pathway in antinociception induced by exercise in rats.
Life Sci
; 86(13-14): 505-9, 2010 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-20153753
ABSTRACT
AIMS:
Physical exercise is responsible for increasing the nociceptive threshold. The present study aimed to investigate the involvement of the nitric oxide/(C)GMP/K(ATP) pathway in antinociception induced by acute aerobic exercise (AAc) in rats. MAINMETHODS:
Wistar rats performed exercise in a rodent treadmill, according to an AAc protocol. The nociceptive threshold was measured by mechanical and thermal nociceptive tests (paw-withdrawal, tail-flick and face-flick). To investigate the involvement of the NO/(C)GMP/K(ATP) pathway the following nitric oxide synthase (NOS) unspecific and specific inhibitors were used N-nitro-l-arginine (NOArg), Aminoguanidine, N(5)-(1-Iminoethyl)-l-ornithine dihydrocloride (L-NIO), N(omega)-Propyl-l-arginine (L-NPA); guanylyl cyclase inhibitor, 1H-[1,2,4]oxidiazolo[4,3-a]quinoxalin-1-one (ODQ); and K(ATP) channel blocker, Glybenclamide; all administered subcutaneously at a dose of 2mg/kg 10min before exercise started. Plasma and cerebrospinal fluid (CSF) nitrite levels were determined by spectrophotometry. KEYFINDINGS:
In the paw-withdrawal, tail-flick and face-flick tests, the AAc protocol produced antinociception, which lasted for more than 15min. This effect was significantly reversed (P<0.05) by NOS specific and unspecific inhibitors, guanylyl cyclase inhibitor (ODQ) and K(ATP) channel blocker (Glybenclamide). Acute exercise was also responsible for increasing nitrite levels in both plasma and cerebrospinal fluid.SIGNIFICANCE:
Taken together, these results suggest that the NO/(C)GMP/K(ATP) pathway participates in antinociception induced by exercise.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
/
Canais de Potássio
/
Transdução de Sinais
/
GMP Cíclico
/
Óxido Nítrico
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article