High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis.
Anal Biochem
; 401(2): 236-41, 2010 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-20211145
ABSTRACT
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Heparina
/
Eletroforese em Gel de Poliacrilamida
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article