An indirect ELISA for serodiagnosis of cattle footrot caused by Fusobacterium necrophorum.
Anaerobe
; 16(4): 317-20, 2010 Aug.
Article
em En
| MEDLINE
| ID: mdl-20304080
ABSTRACT
A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311-644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type A, bovine Escherichia coli, and bovine Salmonella. The rL-ELISA could confirm the existence of antibodies against F. necrophorum at day 7 after infection. Detection of the field samples indicated relative sensitivity of rL-ELISA to nL-ELISA using the purified native leukotoxin A as antigen was 96.43%, and relative specificity of rL-ELISA to nL-ELISA was 94.26%. These data demonstrated the rL-ELISA would have a potential use for early diagnosis of cattle footrot caused by F. necrophorum.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
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Doenças dos Bovinos
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Exotoxinas
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Infecções por Fusobacterium
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Fusobacterium necrophorum
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Anticorpos Antibacterianos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article