Evaluation of a rapid enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis.
J Clin Microbiol
; 48(5): 1716-9, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20335415
ABSTRACT
Diagnosis of strongyloidiasis using stool examination remains unsatisfactory due to the lack of sensitivity and fastidious techniques. In this work, we investigated the value of an anti-Strongyloides IgG enzyme immunoassay (EIA), using a panel of 207 sera retrospectively collected from patients with definitive diagnoses of strongyloidiasis (n=57), other helminthic infections (n=46), eosinophilia without parasitic infection diagnosis (n=54), and digestive disturbances following a tropical journey (n=30) and from 20 negative controls. By following a receiver operating characteristic (ROC) curve analysis, it was possible to optimize the test to reach a sensitivity of 91.2% and a specificity of 93.3%, with 92.8% of patients correctly classified. Considering the incidence of strongyloidiasis diagnosed in our own laboratory, the negative predictive value was calculated at 99.9%. In conclusion, this test is very rapid and easy to perform and may be valuable for diagnosis of strongyloidiasis both in cases where the infection is unrevealed by a parasitological stool examination and in patients at risk for severe clinical forms, such as patients receiving immunosuppressive therapy.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Parasitologia
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Strongyloides
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Estrongiloidíase
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Imunoglobulina G
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Anticorpos Anti-Helmínticos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article