Preparation and antitumor effects of nanovaccines with MAGE-3 peptides in transplanted gastric cancer in mice.
Chin J Cancer
; 29(4): 359-64, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-20346208
ABSTRACT
BACKGROUND AND OBJECTIVE:
As a prospective vaccine carrier, nanoparticles can protect antigens from degradation and enhance immune response. This study prepared nanovaccines with MAGE-3-derived CD4+-CD8+T cell epitope peptides, and investigated its character and antitumor effects on transplanted gastric cancer in mice.METHODS:
We adopted the self-assembly method to prepare peptide/chitosan conjugated with deoxycholic acid (chitosan-deoxycholic acid) nanoparticles. We observed the appearance of the chitosan-deoxycholic acidnanoparticles through a transmission electron microscope (TEM) and analyzed the peptide content and its release pattern by fluorescence spectrophotometry. We observed tumor-suppression efficacy in vivo through animal experiments.RESULTS:
We successfully prepared nanoparticles with MAGE-3 peptide antigen, and its encapsulation efficiency and loading level were about 37% and 17.0%, respectively. These nanoparticles presented a delayed release pattern in phosphate buffered saline (PBS) at pH 7.4, and the full release time was about 48 h. In 2 mg/mL lysozyme, the nanoparticles showed a sudden release, and the full release time was about 24 h. ELISPOT and cytotoxic experiments showed that the MAGE-3 peptide loaded nanoparticles could stimulate immune response in vivo and could generate MAGE-3-targeted cytotoxic T lymphocytes (CTLs), and kill MAGE-3-specific tumor cells. Tumor suppression experiments showed that the regression ratio of the peptide-loaded nanoparticles group was 37.81%.CONCLUSIONS:
MAGE-3 peptide/chitosan-deoxycholic acidvaccine-loaded nanoparticles can stimulate antitumor immune response in vivo and can regress the growth of mouse forestomach carcinoma cell line MFC.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
/
Células Dendríticas
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Linfócitos T Citotóxicos
/
Vacinas Anticâncer
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Antígenos de Neoplasias
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Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article