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Comparison of microbiologic assay methods for hemodialysis fluids.
Arduino, M J; Bland, L A; Aguero, S M; Carson, L; Ridgeway, M; Favero, M S.
Afiliação
  • Arduino MJ; Nosocomial Infections Laboratory Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol ; 29(3): 592-4, 1991 Mar.
Article em En | MEDLINE | ID: mdl-2037676
To help prevent pyrogenic reactions and bacteremia in hemodialysis patients, the Association for the Advancement of Medical Instrumentation and the Centers for Disease Control recommend microbiologic assay of hemodialysis fluids at least monthly. Five commercially available assay systems were evaluated by using the membrane filtration technique with standard methods agar and trypticase soy agar as the standards for comparison. Each assay system was challenged with dialysate and reverse-osmosis water from local dialysis centers, aqueous suspensions of eight laboratory strains of gram-negative bacilli and nontuberculous mycobacteria, and a mixed microbial flora inoculated into reverse-osmosis water and laboratory-prepared dialysate. Mean viable counts from triplicate samples were obtained after incubation at 37 degrees C for up to 72 h. The efficiency of recovery varied with the specific type of microbial challenge. The SPC water sampler (Millipore Corp., Bedford, Mass.) was the most consistent in obtaining the highest viable counts. Other commercial systems were comparable to each other in overall performance. All assay systems tested provided an acceptable balance between microbial recovery and required sampling time, equipment, and expertise.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Soluções para Hemodiálise / Técnicas Bacteriológicas Idioma: En Ano de publicação: 1991 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Soluções para Hemodiálise / Técnicas Bacteriológicas Idioma: En Ano de publicação: 1991 Tipo de documento: Article