Glycolytic enzyme inhibitors affect pancreatic cancer survival by modulating its signaling and energetics.
Anticancer Res
; 30(3): 743-9, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-20392992
ABSTRACT
BACKGROUND AND AIM:
The importance of glycolysis in cancer cells is well documented. The effects of inhibiting glycolysis using metabolic inhibitors iodoacetate (IAA), an inhibitor of GAPDHase, and 3-bromopyruvate (3BP), an inhibitor of hexokinase-II, on survival and signaling of pancreatic cancer cells (Panc-1) were investigated. MATERIALS ANDMETHODS:
Cellular survival was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lactate dehydrogenase (LDH) assay was used to analyze the induced necrosis and protein levels were evaluated using Western blot analysis.RESULTS:
The results show that the inhibitors lowered cellular survival and increased cellular necrosis. Mitogenic signaling pathways were affected by 3BP but not by IAA.CONCLUSION:
We conclude that there may be a cross-talk between signaling pathways and glycolysis in regulating pancreatic cancer cell survival and signaling. Thus, a combination of agents that inhibit both energy production and cell signaling may provide a novel and effective approach to target pancreatic cancer effectively.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Piruvatos
/
Inibidores Enzimáticos
/
Gliceraldeído-3-Fosfato Desidrogenases
/
Hexoquinase
/
Iodoacetatos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article