Low pulmonary expression of epithelial Na(+) channel and Na(+), K(+)-ATPase in newborn infants with congenital diaphragmatic hernia.
Neonatology
; 99(1): 14-22, 2011.
Article
em En
| MEDLINE
| ID: mdl-20588066
ABSTRACT
BACKGROUND:
It has been suggested from several animal studies and clinical observations that congenital diaphragmatic hernia (CDH) with pulmonary hypoplasia is accompanied by a disturbed perinatal ion transport. This could lead to respiratory distress due to slower clearance of fetal lung fluid at birth.OBJECTIVES:
The purpose of this study was to determine whether CDH is related to changes in the expression of three rate-limiting transporter proteins in lung epithelium at birth.METHODS:
Tracheal aspirate was collected from 12 newborn infants with CDH and from 8 newborn control patients. Sampling was performed at postnatal age 18 and at 43 h in the CDH group and at 18 h in the control group. The protein abundance of α-, ß- and γ-epithelial Na(+) channel (ENaC), aquaporin 5 and Na(+), K(+)-ATPase α(1) was analyzed using semiquantitative immunoblotting.RESULTS:
The levels of ß-ENaC, γ-ENaC and Na(+), K(+)-ATPase α(1) collected at 18 h postnatally were significantly lower in CDH infants compared to control infants. In the CDH group, no significant difference in the expression of the ENaC subunits, Na(+), K(+)-ATPase α(1) or aquaporin 5 could be detected between the two sampling time points.CONCLUSIONS:
This downregulation may result in an abnormal lung fluid absorption which could be an important mechanism behind the respiratory distress seen in newborn CDH patients.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
ATPase Trocadora de Sódio-Potássio
/
Mucosa Respiratória
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Aquaporina 5
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Canais Epiteliais de Sódio
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Hérnia Diafragmática
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Pulmão
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article