A compact Acousto-Optic Lens for 2D and 3D femtosecond based 2-photon microscopy.
Opt Express
; 18(13): 13721-45, 2010 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-20588506
ABSTRACT
We describe a high speed 3D Acousto-Optic Lens Microscope (AOLM) for femtosecond 2-photon imaging. By optimizing the design of the 4 AO Deflectors (AODs) and by deriving new control algorithms, we have developed a compact spherical AOL with a low temporal dispersion that enables 2-photon imaging at 10-fold lower power than previously reported. We show that the AOLM can perform high speed 2D raster-scan imaging (>150 Hz) without scan rate dependent astigmatism. It can deflect and focus a laser beam in a 3D random access sequence at 30 kHz and has an extended focusing range (>137 mum; 40X 0.8NA objective). These features are likely to make the AOLM a useful tool for studying fast physiological processes distributed in 3D space.
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Base de dados:
MEDLINE
Assunto principal:
Microscopia Confocal
/
Imageamento Tridimensional
/
Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article