Transcription profiles of aortic smooth muscle cells from atherosclerosis-prone and -resistant regions in young apolipoprotein E-deficient mice before plaque development.
J Vasc Res
; 48(1): 31-42, 2011.
Article
em En
| MEDLINE
| ID: mdl-20606469
ABSTRACT
BACKGROUND/AIMS:
Site-specific atherosclerosis is generally attributed to differential gene expression in endothelial cells. We investigated whether the transcriptome of smooth muscle cells is different between atherosclerosis-prone and atherosclerosis-resistant regions in apolipoprotein E-deficient (apoE-/-) mice before plaque development, and in C57Bl/6 mice.METHODS:
De-endothelialized aortas (both strains 3 males, 3 females, age 4 months) were divided into atherosclerosis-prone (AA ascending aorta, aortic arch and proximal 2 mm of thoracic aorta) and -resistant (CTA central thoracic aorta, i.e. 6 mm distal from the proximal 2 mm) regions. The transcriptome of these two regions was compared using whole-genome mouse microarrays.RESULTS:
Microarray analysis revealed differential expression (>2-fold difference) of 70 and 244 genes in C57Bl/6 and apoE-/- mice. This was confirmed for 6 genes using the real-time quantitative polymerase chain reaction. Up- or downregulation in the AA was observed for 33 and 37 genes in C57Bl/6, and for 186 and 58 genes in apoE-/- mice, respectively. The 201 genes that showed exclusively differential expression in apoE-/- mice were related to atherosclerotic processes, such as cell adhesion, proliferation, differentiation, motility, cell death, lipid metabolism and immune responses.CONCLUSION:
Our findings indicate that smooth muscle cells display an altered transcriptome at atherosclerosis-prone locations before actual lesion development.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Apolipoproteínas E
/
Perfilação da Expressão Gênica
/
Aterosclerose
/
Músculo Liso Vascular
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article