Functional expression of the recombinant ATPase of orf virus.
Arch Virol
; 155(10): 1701-5, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-20652336
ABSTRACT
Nucleotide sequence analysis has indicated that the A32L gene of orf virus can encode an ATPase (Chan et al. in Gene 43244-53, 2009). In this work, we cloned the A32L gene into a prokaryotic expression vector, and the recombinant protein was expressed and purified. The antigenicity of recombinant ATPase was examined by immunoblotting, and its identity was confirmed by mass spectrometry. The ATP hydrolysis function of the purified recombinant protein was examined, and our results showed that it exhibited the ATPase activity. Similar to other viral ATPases, the ATPase of orf virus remained active in the presence of different divalent ions; nevertheless, unlike other viral ATPases, our recombinant ATPase exhibited similar enzymatic activity in reaction buffers of different pH.
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Base de dados:
MEDLINE
Assunto principal:
Vírus do Orf
/
Proteínas Virais
/
Trifosfato de Adenosina
/
Adenosina Trifosfatases
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article