Enhanced expression of adenovirus encoding rhEPO assisted by BAPTA.
Anim Biotechnol
; 21(3): 164-9, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20665288
ABSTRACT
Infection efficiency is the key issue for gene delivery using adenovirus vector and usually unsatisfactory. In this study, recombinant adenoviruses encoding recombinant human EPO were prepared using the Adeasy system, and injected into the mammary gland of goats via the teat canal. BAPTA was used to treat the mammary gland to facilitate adenoviruses infection compared with EGTA. Milk serum was collected from the infected mammary gland and characterized by ELISAs and Western blotting. Expression level of rhEPO from the group treated by BAPTA was higher than that treated by EGTA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cabras
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Adenoviridae
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Eritropoetina
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Ácido Egtázico
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Infecções por Adenoviridae
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Leite
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Glândulas Mamárias Animais
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article