Haemoplasma infection is not a common cause of canine immune-mediated haemolytic anaemia in the UK.
J Small Anim Pract
; 51(10): 534-9, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-21029097
ABSTRACT
OBJECTIVE:
The aim of this study was to investigate whether the two canine haemoplasma species, Mycoplasma haemocanis and "Candidatus Mycoplasma haematoparvum," are commonly associated with immune-mediated haemolytic anaemia (IMHA) in UK dogs.METHODS:
Three groups of dogs were recruited to the study anaemic dogs with primary IMHA (n=37); anaemic dogs not meeting the inclusion criteria for primary IMHA (n=77) and non-anaemic dogs (n=113). DNA was extracted from 100 µl of blood and subjected to real-time quantitative polymerase chain reaction (qPCR) assays for both species of Mycoplasma. Each assay incorporated co-amplification of canine glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous internal control.RESULTS:
Canine GAPDH was successfully amplified by qPCR from all 227 canine blood samples but none contained M. haemocanis or "Candidatus M. haematoparvum" DNA. CLINICALSIGNIFICANCE:
Haemoplasma infection is uncommon in dogs in the UK and no evidence was found that these organisms act as triggers for IMHA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças do Cão
/
Anemia Hemolítica Autoimune
/
Infecções por Mycoplasma
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article