Enantiomeric composition analysis of pranoprofen in equine plasma and urine by chiral liquid chromatography-tandem mass spectrometry in selected reaction monitoring mode.
J Chromatogr B Analyt Technol Biomed Life Sci
; 878(31): 3249-54, 2010 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-21036111
ABSTRACT
The enantioseparation of pranoprofen after its addition in racemic form into equine plasma and urine was conducted by chiral liquid chromatography-tandem mass spectrometry in selected reaction monitoring mode. The methods for the assay of both enantiomers were linear (r≥0.9943) in the low range from 0.001 to 0.1µg/mL and high range from 0.01 to 1.0µg/mL with good precision (% RSD≤5.6) and accuracy (% RE=-5.3 to 1.9). When racemic pranoprofen was orally administered to four horses at a single dose of 3.1mg/kg, the median plasma concentrations of (R)-pranoprofen were lower than the levels of (S)-pranoprofen from start to finish. In contrast, the urinary level of (R)-pranoprofen was 2.5 fold higher than (S)-pranoprofen level for the first 6h, followed by its rapid decrease down below (S)-pranoprofen concentration. Monitoring of the R/S ratios in equine urine may be useful for the prevention of false positive in horse doping test.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Propionatos
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Benzopiranos
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Cromatografia Líquida
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Espectrometria de Massas em Tandem
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Cavalos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article