BECLIN1 from Arabidopsis thaliana under the generic control of regulated expression systems, a strategy for developing male sterile plants.
Plant Biotechnol J
; 8(9): 1005-22, 2010 Dec.
Article
em En
| MEDLINE
| ID: mdl-21050365
ABSTRACT
Induction of male sterility followed by successful outcrossing is a prerequisite for hybrid seed production. In this article, we have identified a novel use of the BECLIN 1 gene of Arabidopsis, in inducing male sterility in plants, when expressed in the anther tapetum of tobacco. We also report a stringently regulated and high-level expression of the desired gene in tapetum by using a two-component transcription regulation system. The tapetum-specific, two-component transcription system utilizes the TGTA-TBPm³ complementation principle that has been demonstrated by us earlier. We also report a glucocorticoid-dependent expression of AtBECLIN 1 in tapetum, thereby developing glucocorticoid-inducible male sterility in plants.
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Base de dados:
MEDLINE
Assunto principal:
Plantas Geneticamente Modificadas
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Arabidopsis
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Proteínas de Arabidopsis
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Proteínas Adaptadoras de Transporte Vesicular
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article