Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila.
Proc Natl Acad Sci U S A
; 107(47): 20553-8, 2010 Nov 23.
Article
em En
| MEDLINE
| ID: mdl-21059961
ABSTRACT
In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartment. We have developed such a marker and show that it is effective and specific in all neuronal populations tested in the peripheral and central nervous system. The marker, which we name DenMark (Dendritic Marker), is a hybrid protein of the mouse protein ICAM5/Telencephalin and the red fluorescent protein mCherry. We show that DenMark is a powerful tool for revealing novel aspects of the neuroanatomy of developing dendrites, identifying previously unknown dendritic arbors, and elucidating neuronal connectivity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Glicoproteínas de Membrana
/
Marcadores Genéticos
/
Dendritos
/
Drosophila melanogaster
/
Proteínas Luminescentes
/
Proteínas do Tecido Nervoso
/
Neurônios
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article