DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damaged chromatin.
Mol Cell
; 40(4): 606-18, 2010 Nov 24.
Article
em En
| MEDLINE
| ID: mdl-21095590
ABSTRACT
When inappropriate DNA structures arise, they are sensed by DNA structure-dependent checkpoint pathways and subsequently repaired. Recruitment of checkpoint proteins to such structures precedes recruitment of proteins involved in DNA metabolism. Thus, checkpoints can regulate DNA metabolism. We show that fission yeast Rad9, a 9-1-1 heterotrimeric checkpoint-clamp component, is phosphorylated by Hsk1(Cdc7), the Schizosaccharomyces pombe Dbf4-dependent kinase (DDK) homolog, in response to replication-induced DNA damage. Phosphorylation of Rad9 disrupts its interaction with replication protein A (RPA) and is dependent on 9-1-1 chromatin loading, the Rad9-associated protein Rad4/Cut5(TopBP1), and prior phosphorylation by Rad3(ATR). rad9 mutants defective in DDK phosphorylation show wild-type checkpoint responses but abnormal DNA repair protein foci and decreased viability after replication stress. We propose that Rad9 phosphorylation by DDK releases Rad9 from DNA damage sites to facilitate DNA repair.
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Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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Dano ao DNA
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Cromatina
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Ciclo Celular
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Proteínas Serina-Treonina Quinases
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Proteínas de Ciclo Celular
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Proteínas de Schizosaccharomyces pombe
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article