Efficient production of uridine 5'-diphospho-N-acetylglucosamine by the combination of three recombinant enzymes and yeast cells.
Prep Biochem Biotechnol
; 40(4): 294-304, 2010.
Article
em En
| MEDLINE
| ID: mdl-21108133
ABSTRACT
Uridine 5'-diphospho N-acetylglucosamine (UDP-GlcNAc) is an important nucleotide sugar in the biochemistry of all living organisms, and it is an important substrate in the synthesis of oligosaccharides. In the present work, three bioactive enzymes, namely, glucokinase (YqgR), GlcNAc-phosphate mutase (Agm1), and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU), were produced effectively as soluble form in recombinant Escherichia coli. These three enzymes and dried yeast together were used to construct a multistep enzymatic system, which could produce UDP-GlcNAc efficiently with N-acetylglucosamine (GlcNAc) as the substrate. After the optimization of various reaction conditions, 31.5 mMUDP-GlcNAc was produced from 50 mMGlcNAc and 50 mMUMP.
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1
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Bacillus subtilis
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Uridina Difosfato N-Acetilglicosamina
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Microbiologia Industrial
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Proteínas Recombinantes
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Escherichia coli
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article