Use of in vitro technologies to study phase II conjugation in equine sports drug surveillance.
Bioanalysis
; 2(12): 1971-88, 2010 Dec.
Article
em En
| MEDLINE
| ID: mdl-21110741
ABSTRACT
BACKGROUND:
Within equine drug surveillance, there is significant interest in analyzing intact phase II conjugates of drugs in urine, but progress has been limited by a lack of reference material.METHOD:
In this study, in vitro techniques using equine liver fractions were employed to produce glucuronide and sulfate conjugates of stanozolol, 16ß-hydroxystanozolol and nandrolone, the glucuronide conjugate of morphine and the glutathione metabolite of chlordinitrobenzene for the first time in equine sports drug surveillance.RESULTS:
The glucuronide conjugate of the synthetic progestagen altrenogest was also produced in vitro, removing the requirement for sample hydrolysis during routine urinalyses.CONCLUSION:
These results highlight the potential of in vitro studies for the production of phase II reference material, allowing the development of assays based on intact conjugates.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Esteroides
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Detecção do Abuso de Substâncias
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Glucuronídeos
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Dopagem Esportivo
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Glutationa
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Anabolizantes
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Fígado
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article