[DNA barcoding in medicinal plants Caprifoliaceae].
Zhongguo Zhong Yao Za Zhi
; 35(19): 2527-32, 2010 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-21174758
ABSTRACT
OBJECTIVE:
To determine the candidate sequences which can be used as DNA barcode to identify species in Caprifoliaceae family by screening out from four different DNA fragments sequences.METHOD:
PCR amplification, sequencing efficiency, differential intra- and interspecific divergences, the DNA barcoding gap and identification efficiency were used to evaluate these loci.RESULT:
The ITS2 was used as a candidate sequence of DNA barcode to identify the species in Caprifoliaceae family, whose rate of success in identification in genera level was 100% and in species 96.6%, and psbA-trnH as a complementary barcode to ITS2 for Caprifoliaceae.
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Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Especificidade da Espécie
/
DNA de Plantas
/
Caprifoliaceae
/
Identificação Psicológica
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article