Pulsed-field gel electrophoresis genotyping of Taylorella equigenitalis isolates collected in the United States from 1978 to 2010.
J Clin Microbiol
; 49(3): 829-33, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21191049
ABSTRACT
Taylorella equigenitalis is the etiologic agent of contagious equine metritis (CEM), a venereal disease of horses. A total of 82 strains of T. equigenitalis isolated in the United States were analyzed by pulsed-field gel electrophoresis (PFGE) after digestion of genomic DNA with restriction enzyme ApaI. Twenty-eight of those strains isolated from horses in the 2009 U.S. outbreak (CEM09) were further analyzed with NotI and NaeI enzymes. When ApaI alone was used for analysis, the 82 isolates clustered into 15 different genotypes that clearly defined groups of horses with known epidemiological connections. The PFGE profiles of the CEM09 isolates were indistinguishable after digestion with ApaI, NotI, and NaeI and did not match those of isolates from previous U.S. outbreaks in 1978 and 2006 or of any other isolate from the National Veterinary Services Laboratories (NVSL) culture library. Coupled with the fact that the CEM09 isolates are epidemiologically related, these results suggest a common source for the outbreak not linked to previous occurrences of CEM in the United States.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Tipagem Bacteriana
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Infecções por Bactérias Gram-Negativas
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Eletroforese em Gel de Campo Pulsado
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Taylorella equigenitalis
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Doenças dos Cavalos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article