Purification of human C-reactive protein by immunoaffinity chromatography using mouse monoclonal antibody.
J Biochem Biophys Methods
; 21(1): 75-80, 1990 Jun.
Article
em En
| MEDLINE
| ID: mdl-2120317
ABSTRACT
Human C-reactive protein (CRP) was purified from sera with a high concentration of CRP by immunoaffinity chromatography using mouse monoclonal antibody (mAb) to CRP (No. 18) and ion exchange on DEAE-Sephacel. CRP bound to the immunoaffinity column was eluted by 0.5 M acetate buffer, pH 5.0 containing 1.5 M NaCl. Final recovery of CRP was 90%. No significant immunochemical differences were observed between CRP obtained by the immunoaffinity chromatography and CRP purified by the method of Hokama and Riley.
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Base de dados:
MEDLINE
Assunto principal:
Proteína C-Reativa
/
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article