CdTe quantum dots as a highly selective probe for prion protein detection: colorimetric qualitative, semi-quantitative and quantitative detection.
Talanta
; 83(5): 1716-20, 2011 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-21238774
ABSTRACT
CdTe quantum dots (QDs) were used as a highly selective probe for the detection of prion protein. Orange-emitting precipitates appeared within 30s of the addition of recombination prion protein (rPrP) to a solution of green-emitting CdTe QDs. This allowed colorimetric qualitative and semi-quantitative detection of rPrP. The decrease in fluorescence intensity of the supernatant could be used for quantitative detection of rPrP. The fluorescence intensity of the supernatant was inversely proportional to the rPrP concentration from 8 to 200 nmol L(-1) (R(2)=0.9897). Transmission electron microscopy results showed that fibrils existed in the precipitates and these were partly transformed to amyloid plaques after the addition of rPrP.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
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Telúrio
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Príons
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Compostos de Cádmio
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Pontos Quânticos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article