[HDL inhibit cytokine production in a mouse model of urate crystal-induced inflammation]. / Le HDL inibiscono la produzione di citochine nel modello murino di infiammazione da microcristalli di urato monosodico.
Reumatismo
; 62(4): 266-72, 2010.
Article
em It
| MEDLINE
| ID: mdl-21253620
ABSTRACT
OBJECTIVES:
To evaluate whether high density lipoproteins (HDL) affect monosodium urate (MSU) crystal-induced inflammation in the murine air pouch model.METHODS:
MSU crystals were prepared by Denko's method and sterilized by heating at 180°C for 2 h before each experiment. Human HDL were isolated from peripheral blood of healthy volunteers. MSU crystals (2 mg in 1 ml of PBS) were injected into subcutaneous air pouches in mice in the presence or absence of HDL (0.1 mg). Negative control pouches received 1 ml of PBS. To recover pouch fluid, the pouches were washed with 2 ml of PBS after the animals were sacrificed. The leukocyte count in the lavage fluids was obtained using a hemocytometer and differential leukocyte count was determined by May-Grunwald-Giemsa staining. IL-6, KC, CCL2 and TNF-α levels were measured in exudates by ELISA.RESULTS:
MSU crystals increased the number of leukocytes and the neutrophil migration, as well as the concentrations of IL-6, KC and CCL2 in pouch fluids, while the TNF-α levels were not detectable. The treatment with HDL led to a reduction in all inflammatory parameters the leukocyte count decreased by 73%; the neutrophil density decreased by 35%; the IL-6, KC and CCL2 concentration decreased by 4-, 6- and 5-fold respectively.CONCLUSIONS:
This study shows that HDL may limit the inflammatory process by inhibiting leukocyte recruitment and cytokine release. HDL are likely to represent a mechanism of control of crystal-induced inflammation.
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Base de dados:
MEDLINE
Assunto principal:
Citocinas
/
Modelos Animais de Doenças
/
Inflamação
/
Lipoproteínas HDL
Idioma:
It
Ano de publicação:
2010
Tipo de documento:
Article