A sensitive LC-MS/MS method for determination of levamisole in human plasma: application to pharmacokinetic study.
J Chromatogr B Analyt Technol Biomed Life Sci
; 879(5-6): 299-303, 2011 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-21256094
ABSTRACT
A sensitive and rapid LC-MS/MS method was developed and validated for the determination of levamisole in human plasma. The assay was based on liquid-liquid extraction of analytes from human plasma with ethyl ether. Chromatographic separation was carried on an Agilent HC-C(8) column (150 mm × 4.6 mm, 5 µm) at 40°C, with a mobile phase consisting of acetonitrile-10 mM ammonium acetate (7030, v/v), a flow rate of 0.5 mL/min and a total run time of 6 min. Detection and quantification were performed by mass spectrometry in the multiple reaction monitoring mode with positive electrospray ionization m/z at 205.1â178.2 for levamisole, and m/z 296.1â264.1 for mebendazole (internal standard). The assay was linear over a concentration range of 0.1-30 ng/mL with a lower limit of quantification of 0.1 ng/mL. The coefficient of variation of the assay precision was less than 8.5%. The assay was successfully used to analyze human plasma samples in a pharmacokinetic study where levamisole was administered as a liniment.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Levamisol
/
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article