Ultratrace enrichment of tyrosine phosphorylated peptides on an imprinted polymer.
Anal Chem
; 83(5): 1862-5, 2011 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-21306124
ABSTRACT
Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO(2)-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Polímeros
/
Tirosina
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article