The functional expression of extracellular calcium-sensing receptor in rat pulmonary artery smooth muscle cells.
J Biomed Sci
; 18: 16, 2011 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-21314926
ABSTRACT
BACKGROUND:
The extracellular calcium-sensing receptor (CaSR) belongs to family C of the G protein coupled receptors. Whether the CaSR is expressed in the pulmonary artery (PA) is unknown.METHODS:
The expression and distribution of CaSR were detected by RT-PCR, Western blotting and immunofluorescence. PA tension was detected by the pulmonary arterial ring technique, and the intracellular calcium concentration ([Ca2+]i) was detected by a laser-scanning confocal microscope.RESULTS:
The expressions of CaSR mRNA and protein were found in both rat pulmonary artery smooth muscle cells (PASMCs) and PAs. Increased levels of [Ca2+]o (extracellular calcium concentration) or Gd3+ (an agonist of CaSR) induced an increase of [Ca2+]i and PAs constriction in a concentration-dependent manner. In addition, the above-mentioned effects of Ca2+ and Gd3+ were inhibited by U73122 (specific inhibitor of PLC), 2-APB (specific antagonist of IP3 receptor), and thapsigargin (blocker of sarcoplasmic reticulum calcium ATPase).CONCLUSIONS:
CaSR is expressed in rat PASMCs, and is involved in regulation of PA tension by increasing [Ca2+]i through G-PLC-IP3 pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Artéria Pulmonar
/
Receptores de Detecção de Cálcio
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article