Novel high-affinity Aß-binding peptides identified by an advanced in vitro evolution, progressive library method.
Protein Pept Lett
; 18(6): 642-50, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21342094
ABSTRACT
Recent studies have been supporting that the generation of Aß42 oligomers is responsible for Alzheimer's disease. Therefore, those peptides which bind to Aß42 are scientifically interesting and can be possible candidates for the diagnosis and therapy of Alzheimer's disease. A systemic in vitro evolution, developed recently and the designated progressive library method (PLM), was applied to obtain Ab42-binding aptamers peptides. As a result, high affinity peptide aptamers made of 8 or 9 amino acids could be identified by this approach, endorsing the methodological effectiveness. Namely, the selection products from the secondary library of diversified peptides, which was constructed based on the information obtained from the primary library selection, were confirmed to be superior to those selected from the primary library as had been reported previously. The affinities of those peptides measured by SPR (surface plasmon resonance) were comparable to or higher than that of those peptides so far reported (K(d) of 10â»7). The other peptides selected were confirmed of their binding by a novel mode of gel shift assay (fluorescence enhancement caused by the binding). Thus, novel Aß42-binding peptides with high affinity were provided for the future Alzheimer's disease study. The demonstration of the effectiveness of the systemic in vitro evolution of PLM is very encouraging for the study of identifying novel functional peptides.
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Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Peptídeos beta-Amiloides
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Evolução Molecular Direcionada
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Biblioteca de Peptídeos
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Aptâmeros de Peptídeos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article