Spectral-domain phase microscopy with improved sensitivity using two-dimensional detector arrays.
Rev Sci Instrum
; 82(2): 023706, 2011 Feb.
Article
em En
| MEDLINE
| ID: mdl-21361600
ABSTRACT
In this work we demonstrate the use of two-dimensional detectors to improve the signal-to-noise ratio (SNR) and sensitivity in spectral-domain phase microscopy for subnanometer accuracy measurements. We show that an increase in SNR can be obtained, from 82 dB to 105 dB, using 150 pixel lines of a low-cost CCD camera as compared to a single line, to compute an averaged axial scan. In optimal mechanical conditions, phase stability as small as 92 µrad, corresponding to 6 pm displacement accuracy, could be obtained. We also experimentally demonstrate the benefit of spatial-averaging in terms of the reduction of signal fading due to an axially moving sample. The applications of the improved system are illustrated by imaging live cells in culture.
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Base de dados:
MEDLINE
Assunto principal:
Microscopia
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article