Activation and differentiation of mesenchymal stem cells.
Methods Mol Biol
; 717: 245-53, 2011.
Article
em En
| MEDLINE
| ID: mdl-21370035
ABSTRACT
Mesenchymal stem cells (MSCs) are multipotent cells and exhibit two main characteristics that define stem cells self-renewal and differentiation. MSCs can migrate to sites of injury, inflammation, and tumor. Moreover, MSCs undergo myofibroblast-like differentiation, including increased production of alpha smooth muscle actin (α-SMA) in response to transforming growth factor-ß (TGF-ß), a growth factor commonly secreted by tumor cells to evade immune surveillance. Based on our previous finding, hMSCs become activated and resemble carcinoma-associated myofibroblasts upon prolonged exposure to conditioned medium from MDAMB231 human breast cancer cells. Here, we show that keratinocyte-conditioned medium (KCM) induces differentiation of MSCs to resemble dermal myofibroblast-like cells using immunofluorescence techniques demonstrating punctate vinculin staining, and F-actin filaments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunofluorescência
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article