Nine crystal structures determine the substrate envelope of the MDR HIV-1 protease.
Protein J
; 30(3): 173-83, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21394574
ABSTRACT
Under drug selection pressure, emerging mutations render HIV-1 protease drug resistant, leading to the therapy failure in anti-HIV treatment. It is known that nine substrate cleavage site peptides bind to wild type (WT) HIV-1 protease in a conserved pattern. However, how the multidrug-resistant (MDR) HIV-1 protease binds to the substrate cleavage site peptides is yet to be determined. MDR769 HIV-1 protease (resistant mutations at residues 10, 36, 46, 54, 62, 63, 71, 82, 84, and 90) was selected for present study to understand the binding to its natural substrates. MDR769 HIV-1 protease was co-crystallized with nine substrate cleavage site hepta-peptides. Crystallographic studies show that MDR769 HIV-1 protease has an expanded substrate envelope with wide open flaps. Furthermore, ligand binding energy calculations indicate weaker binding in MDR769 HIV-1 protease-substrate complexes. These results help in designing the next generation of HIV-1 protease inhibitors by targeting the MDR HIV-1 protease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Protease de HIV
/
HIV-1
/
Farmacorresistência Viral Múltipla
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article