[Experiment studies on growth and proliferation of Hep-2 cells by silence of TRF2 gene].
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
; 24(24): 1131-5, 2010 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-21395184
ABSTRACT
OBJECTIVE:
To investigate the effect of RNA interference silencing telomere repeat factor 2 by observing Hep-2 cells' proliferation and apoptosis in larynx carcinoma cell line.METHOD:
A recombinant plasmid containing a single shRNA (shTRF2) was constructed. The expression of TRF2 gene was detected by PCR and cell proliferation was examined using CCK-8. Hep-2 cells' apoptosis was detected by flow cytometer (FCM).RESULT:
After treatment with shTRF2, the expression of TRF2 was distinctively depressed, and Hep-2 cells proliferation was obviously inhibited. Compared with control and negative group, cells with treatment of RNAi exhibited significantly more apoptosis.CONCLUSION:
Using RNA interference technique to silence TRF2 gene is effective on inhibiting cancer cells' proliferation and help to induce cancer cells' apoptosis.
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Base de dados:
MEDLINE
Assunto principal:
Proteína 2 de Ligação a Repetições Teloméricas
/
RNA Interferente Pequeno
/
Interferência de RNA
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article