Methyl-coenzyme M reductase from Methanothermobacter marburgensis.
Methods Enzymol
; 494: 159-87, 2011.
Article
em En
| MEDLINE
| ID: mdl-21402215
ABSTRACT
Methyl-coenzyme M reductase catalyzes the reversible synthesis of methane from methyl-coenzyme M in methanogenic and ANME-1 and ANME-2 Archaea. The purification procedure for methyl-coenzyme M reductase from Methanothermobacter marburgensis is described. The procedure is an accumulation of almost 30 years of research on MCR starting with the first purification described by Ellefson and Wolfe (Ellefson, W.L., and Wolfe, R.S. (1981). Component C of the methylreductase system of Methanobacterium. J. Biol. Chem.256, 4259-4262). To provide a context for this procedure, some background information is provided, including a description of whole cell experiments that provided much of our knowledge of the behavior and properties of methyl-coenzyme M reductase.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Methanobacteriaceae
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article