A striking common O-linked N-acetylglucosaminyl moiety between cruzipain and myosin.
Parasite Immunol
; 33(7): 363-70, 2011 Jul.
Article
em En
| MEDLINE
| ID: mdl-21426361
ABSTRACT
Single units of O-linked N-acetylglucosamine (GlcNAc), usually components of nuclear and cytoplasmatic proteins, are present at the C-terminal domain of cruzipain (Cz), a lysosomal major antigen from Trypanosoma cruzi. On the other hand, antibodies directed against some self-antigens like myosin are associated with Chagas heart disease. The participation of O-GlcNAc moieties in the molecular antigenicity of Cz was determined using GlcNAc linked to aprotinin by ELISA. The immune cross-reactivity between Cz and myosin is mainly focused in the C-T domain. ELISA inhibition assays using rabbit sera specific for Cz and C-T in conjunction with immune-gold electron microscopy analysis of heart tissues from mice immunized with C-T confronted with polyclonal rabbit sera specific for Cz and C-T prior and after myosin adsorption provided evidence which indicates that O-GlcNAc moieties constitute a common epitope between Cz and either myosin or other cardiac O-GlcNAc-containing proteins, showing a new insight into the molecular immune pathogenesis of Chagas heart disease.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Acetilglucosamina
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Trypanosoma cruzi
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Anticorpos Antiprotozoários
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Cisteína Endopeptidases
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Miosinas
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Reações Cruzadas
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Epitopos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article