[Differentiation of genetic variants of Crimean-Congo hemorrhagic fever virus].
Vopr Virusol
; 56(1): 30-3, 2011.
Article
em Ru
| MEDLINE
| ID: mdl-21427952
ABSTRACT
The paper describes a simple, rapid screening of samples potentially containing Crimean-Congo hemorrhagic fever (CCHF) virus strains, by applying the restriction analysis of amplicones, for the differentiation of CCHF virus genotypes that are characteristic of Europe from virus biovariants uncharacteristic of this area, this technique requiring no sequence at the first stage. For this screening, the authors propose to use the PCR fragment of CCHF L segment that comprises a variable region, as well as Alul and Haelll restriction endonucleases. The screening scheme proposed for samples potentially containing CCHF virus may aid investigators to monitor in order to detect uncharacteristic genotypic virus variants in the Russian Federation and other European countries.
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Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
Mapeamento por Restrição
/
Vírus da Febre Hemorrágica da Crimeia-Congo
/
Febre Hemorrágica da Crimeia
Idioma:
Ru
Ano de publicação:
2011
Tipo de documento:
Article