Tracking down of laryngo-pharyngeal metastasis.
Roum Arch Microbiol Immunol
; 69(3): 153-63, 2010.
Article
em En
| MEDLINE
| ID: mdl-21434592
ABSTRACT
BACKGROUND:
In Romania, the most optimistic statistics give a 5 years survival rate in approximately 33% of laryngo-pharyngeal cancer patients. Considering that a cell carrying the viral DNA is originating from primary tumor, we have tested whether HPV DNA could be detected in the blood cell of patients with laryngeal cancer as a marker of disease progression and metastases.METHODS:
The study was performed on 85 patients (59 +/- 8.7 age) with laryngo-pharyngeal cancer. HPV DNA was detected in tumor using nested PCR with consensus primers, and also in local lymph nodes and/or blood cells from patients HPV positive in primary tumor.RESULTS:
HPV DNA was detected in 75.29% of analyzed tumours, and all HPV16 positive samples were confirmed by mRNA E6 expression. 56.3% of patients presented HPV DNA in peripheral circulation as confirmed by PCR with E6 HPV16 specific primers followed by Southern Blot.CONCLUSION:
Our results sustain that the detection of HPV DNA in blood is a "surrogate marker" of metastasis when extension of metastasis cannot be estimated, this observation is very important for management of cancer patients with laryngopharyngeal localization.
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Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Neoplasias Laríngeas
/
Infecções por Papillomavirus
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article