Expression of a recombinant chimeric protein of hepatitis A virus VP1-Fc using a replicating vector based on Beet curly top virus in tobacco leaves and its immunogenicity in mice.
Plant Cell Rep
; 30(8): 1513-21, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21442402
ABSTRACT
We describe the expression and immunogenicity of a recombinant chimeric protein (HAV VP1-Fc) consisting of human hepatitis A virus VP1 and an Fc antibody fragment using a replicating vector based on Beet curly top virus (BCTV) in Agrobacterium-infiltrated Nicotiana benthamiana leaves. Recombinant HAV VP1-Fc was expressed with a molecular mass of approximately 68 kDa. Recombinant HAV VP1-Fc, purified using Protein A Sepharose affinity chromatography, elicited production of specific IgG antibodies in the serum after intraperitoneal immunization. Following vaccination with recombinant HAV VP1-Fc protein, expressions of IFN-γ and IL-4 were increased in splenocytes at the time of sacrifice. Recombinant VP1-Fc from infiltrated tobacco plants can be used as an effective experimental immunogen for research into vaccine development.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus de Plantas
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Nicotiana
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Fragmentos Fc das Imunoglobulinas
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Proteínas Estruturais Virais
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Vetores Genéticos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article