Heme oxygenase-1 transgenic overexpression did not prevent artery injury induced by electric stimulation and pressure overload in mice.
Eur J Pharmacol
; 659(2-3): 199-205, 2011 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-21458444
ABSTRACT
Heme oxygenase-1 (HO-1) shows multiple beneficial effects on cardiovascular diseases. However, the effect of HO-1 on the injury of artery has never been identified. In the present study, we established systemic HO-1 overexpression transgenic mice and investigated the effect of HO-1 on the injury of artery induced by electric stimulation and pressure-overload in transgenic mice. Artery injury was induced by electric stimulation and pressure overload. The contractive function, endothelium-dependent and -independent relaxation of arteries were measured through an isometric force transducer connected to a multichannel acquisition and analysis system. Western blot results showed that HO-1 protein level in transgenic mice arteries was significantly higher than that in wild type mice arteries, while no difference of HO-2 protein level in the arteries of transgenic and wild type mice. Arterial reendothelialization after electric injury was accelerated in transgenic mice. No significant difference in contractive function, endothelium-dependent and -independent relaxation of arteries was observed between wild type and transgenic mice at day 7 after electric injury and 4 weeks after pressure overload. We concluded that HO-1 overexpression accelerated the reendothelialization, but did not prevent the functional impairment of injured artery in mice.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Artérias
/
Pressão
/
Transgenes
/
Heme Oxigenase-1
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article