Heme oxygenase-1 transgenic overexpression did not prevent artery injury induced by electric stimulation and pressure overload in mice.

ABSTRACT

Heme oxygenase-1 (HO-1) shows multiple beneficial effects on cardiovascular diseases. However, the effect of HO-1 on the injury of artery has never been identified. In the present study, we established systemic HO-1 overexpression transgenic mice and investigated the effect of HO-1 on the injury of artery induced by electric stimulation and pressure-overload in transgenic mice. Artery injury was induced by electric stimulation and pressure overload. The contractive function, endothelium-dependent and -independent relaxation of arteries were measured through an isometric force transducer connected to a multichannel acquisition and analysis system. Western blot results showed that HO-1 protein level in transgenic mice arteries was significantly higher than that in wild type mice arteries, while no difference of HO-2 protein level in the arteries of transgenic and wild type mice. Arterial reendothelialization after electric injury was accelerated in transgenic mice. No significant difference in contractive function, endothelium-dependent and -independent relaxation of arteries was observed between wild type and transgenic mice at day 7 after electric injury and 4 weeks after pressure overload. We concluded that HO-1 overexpression accelerated the reendothelialization, but did not prevent the functional impairment of injured artery in mice.