Plasma biomarker discovery using 3D protein profiling coupled with label-free quantitation.
Methods Mol Biol
; 728: 3-27, 2011.
Article
em En
| MEDLINE
| ID: mdl-21468938
ABSTRACT
In-depth quantitative profiling of human plasma samples for biomarker discovery remains quite challenging. One promising alternative to chemical derivatization with stable isotope labels for quantitative comparisons is direct, label-free, quantitative comparison of raw LC-MS data. But, in order to achieve high-sensitivity detection of low-abundance proteins, plasma proteins must be extensively pre-fractionated, and results from LC-MS runs of all fractions must be integrated efficiently in order to avoid misidentification of variations in fractionation from sample to sample as "apparent" biomarkers. This protocol describes a powerful 3D protein profiling method for comprehensive analysis of human serum or plasma proteomes, which combines abundant protein depletion and high-sensitivity GeLC-MS/MS with label-free quantitation of candidate biomarkers.
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1
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores
/
Proteômica
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article