Frozen water phase method for logD measurement using a 96-well plate.
Talanta
; 84(3): 809-13, 2011 May 15.
Article
em En
| MEDLINE
| ID: mdl-21482286
ABSTRACT
In this study, a frozen water phase method for logD measurement using a 96-well plate was developed. In the case of logD measurement of compounds, the problem of octanol contamination often occurs; in lipophilic compounds, the concentration of the octanol phase is much higher than that of the water phase. When the water phase is separated from the octanol phase, a small amount of octanol phase contamination could strongly influence the concentration of the water phase. To avoid this problem, the frozen water phase method was developed. The water phase was frozen in liquid nitrogen and then the unfrozen octanol phase was removed. To remove the portion of the octanol remaining on the frozen water phase, the surface of the frozen water phase was washed with octanol and water/ethanol (50/50, v/v). The validity of the method was confirmed by results of commercially available drugs at the logD range from 0 to 4. Further, it was found that this method had the ability to evaluate the pH-logD profile of compounds in the range from pH 2 to pH 12. As a result, we developed the convenient and accurate method that is effective in preventing contamination with a wide dynamic range.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Água
/
Congelamento
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article