A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa.
Vet Res
; 42: 58, 2011 Apr 14.
Article
em En
| MEDLINE
| ID: mdl-21492440
ABSTRACT
Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pseudorraiva
/
Doenças dos Suínos
/
Membrana Basal
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Herpesvirus Suídeo 1
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Serina Proteases
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Mucosa Nasal
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article