Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 67(Pt 4): 461-3, 2011 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-21505241
ABSTRACT
Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4â
Å resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3â
Å, and is estimated to contain between six and 12 molecules per asymmetric unit.
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Base de dados:
MEDLINE
Assunto principal:
Sirtuína 1
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article