Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability.
Environ Microbiol
; 13(5): 1357-69, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-21507178
ABSTRACT
We report a study of the role of putative exopolysaccharide gene clusters in the formation and stability of Pseudomonas putida KT2440 biofilm. Two novel putative exopolysaccharide gene clusters, pea and peb, were identified, and evidence is provided that they encode products that stabilize P. putida KT2440 biofilm. The gene clusters alg and bcs, which code for proteins mediating alginate and cellulose biosynthesis, were found to play minor roles in P. putida KT2440 biofilm formation and stability under the conditions tested. A P. putida KT2440 derivative devoid of any identifiable exopolysaccharide genes was found to form biofilm with a structure similar to wild-type biofilm, but with a stability lower than that of wild-type biofilm. Based on our data, we suggest that the formation of structured P. putida KT2440 biofilm can occur in the absence of exopolysaccharides; however, exopolysaccharides play a role as structural stabilizers.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
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Família Multigênica
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Pseudomonas putida
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Biofilmes
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article